Summarize the long term goals of research in which you participated:
There is 2 research that was conducted:
1. Background – HeLa cells task: cell passaging, making mediums such as DMEM, aseptic technique usage, cell adhering, hemocytometer cell counting. Usage of inverted microscopes, cell dyes, and fluorescence microscopes.
Long term goals – The ability to use non-lethal chemicals and substances to induce apoptosis via Bcl-2 genes in HeLa cells. Finding and using proper lethal doses to obtain the highest apoptosis rate of the cancer cells without the usage of harmful chemicals.
Background- Using CRISPR cas 9 deletions of viral receptors deleting viral receptors for herpes simplex virus 1 and enterovirus 7. We infected healthy cells from donors in our class with herpes simplex virus to create a personal cell line for our specific usage. Due to Crispr Cas 9 being the mode of entry, we designed our plasmids and forward/reverse primers.
Long-term goals – Using CRISPR gene editing to remove viral receptors on healthy cells to prevent the viruses from attaching to the surface, preventing infection altogether.
Program of research that excites me: Crispr system of gene editing has fascinated me ever since my encounter with it in the research lab. To create platform algae from which insert of certain genes allows it to conduct tasks that can tackle today’s and future problems. Green algae are the largest producer of oxygen on the planet, by changing genes that effect
