Describe microarray experiments carried out by Lim et al. (2005) to prove that mir-124 is important for defining brain specific gene expression pattern and that the 5’-end of this miRNA is sufficient for its function.

miR-124

Essay Question: miR-124 is a microRNA (miRNA) that is preferentially expressed in the brain. Briefly, summarise how miRNAs mediate post-transcriptional repression. Also, describe microarray experiments carried out by Lim et al. (2005) to prove that mir-124 is important for defining brain specific gene expression pattern and that the 5’-end of this miRNA is sufficient for its function.

Refer to following research paper: Lim et al. (2005) Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. doi: 10.1038/nature03315.