Interpret and follow an extended set of lab protocols over 4 days, preparing and organising a set of practical activities, requiring time management and personal planning and to design and complete further experiments, as required, depending on the experimental results obtained.

AKR1C3 lab report

Purify an over-expressed recombinant protein (AKR1C3) from bacteria, determine its purity, complete initial, then more detailed kinetic characterisation of the protein purified.

Explore the kinetic properties of an unknown ligand against the purified protein.

Interpret and follow an extended set of lab protocols over 4 days, preparing and organising a set of practical activities, requiring time management and personal planning and to design and complete further experiments, as required, depending on the experimental results obtained.

Record experimental lab data in a lab book in an organised and self-explanatory style with sufficient detail, so that another biochemist could understand the experiments done, the data collected, the analysis used, and repeat the experiments getting the same or similar results. Some useful tips and guidance is available in this article: https://www.sciencemag.org/careers/2019/09/how-keep-lab-notebook

Write a short scientific paper (“the AKR Report”) to analyse and interpret the experimental data collected, list the aims of the experiments, introduce the background information, summarise the methodology, report the experimental results and the quality of the data by the appropriate statistical methods, and discuss the experimental outcomes within the context of the wider literature.