FLOW CYTOMETRY APPLICATIONS FOR LEUKAEMIA DIAGNOSIS
Provide diagnosis of one of the types of leukaemia based on the bone marrow morphology and immunophenotyping of the bone marrow, leukaemic cells shown in red colour.
You need to indicate the lineage and the subtype. Explain the reasons for the diagnosis, (word limit 2,000, interval 1.5, exclusive of any illustrations and of the reference list).
For this, characterise the level of positivity of each of the markers and its significance, and compare the conclusions to the microscopy data.
You will need to identify the meaning of each of the phenotypic characteristics given in the slides with the assistance of the file provided (normal bone marrow) and your own research.
Indicative marking scheme:
1. Justified accurate basic diagnosis of the type of leukaemia (lineage) – 50- 59%;
2. Justified accurate diagnosis of the type of leukaemia, with the correct subtype and explanation of the importance of isotype controls,– 60- 69%;
3. All of the above plus differential
diagnosis, treatment and prognosis. > 70%.
