What important discovery does this paper make about TAF-4 and HIF-1? In worms, approximately how many genes does HIF-1 regulate during hypoxia? What happens to the longevity of worms when RNAi is used to knockdown HIF-1 in MIT mutant worms?

Hypoxia – TAF-4 is required for the life extension of isp-1, clk-1 and tpk-1 Mit mutants

Instructions:

Answer questions 1-4 by reading the following article. All answers must come from SOLELY from the following journal article . The answers should be 1-2 sentence and it should summarize the answers EFFECTIVELY and CONCISELY in 1-2 sentences unless specified otherwise. In question 1, you may use 4-5 sentences. Make sure the answers are accurate and precise and derived appropriately from the paper. No specific format needs to be use, only the answers need to be given. No citations required.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838777/

1. (2%) Explain the relevance of testing the phenotype of RNAi treatment for 400 genes on wild type or MIT mutant c. elegans (make sure to address why MIT mut worms were used and what research group(s) discovered this particular MIT mut worm phenotype).

2. (1%) What important discovery does this paper make about TAF-4 and HIF-1?

3. (1%) In worms, approximately how many genes does HIF-1 regulate during hypoxia?

4. (1%) What happens to the longevity of worms when RNAi is used to knockdown HIF-1 in MIT mutant worms?