Post lab questions
3. Refer back to the picture you drew in your data section of the cells you see in the preparation of your gumline slide.
a. There are some eukaryotic cells. How do you know that these are eukaryotic cells and not prokaryotic? List at least two reasons.
b. Explain why eukaryotic cells are not considered gram-positive or gram-negative cells.
c. While they might not be gram positive or negative, do the eukaryotic cells stain like a gram-positive cell or a gram-negative cell? Does your result on your gram stain make sense based on what you know about the gram stain procedure and the difference between eukaryotic and prokaryotic cells?
4. Both acid fast and non-acid fast cells are stained by carbolfttchsin. How is it, then, that we can use it as a differential stain?
5. Explain how an acid-fast cell wall is different than a gram negative or gram-positive cell wall.