Describe the controls used in the starch test (Activity 1). In the quick test for fats (Activity 3), is there a relationship between the size of the spot and the fat content of the food? Explain your answer. In which test tube(s) did protein digestion occur (Activity 4)? How do you know?

Biological Macromolecules and Enzymes

Data

Activity 1

Data Table 1

Test Tube ID	Observations
Test tube 1 Water + iodine potassium iodide
Test tube 2 Water + potato starch + iodine potassium iodide
Test tube 3 Water + albumin solution + iodine potassium iodide

 

1. Describe the controls used in the starch test (Activity 1).

Activity 2

Data Table 2

Test Tube ID	Observations
Test tube 1 Water + Biuret reagent
Test tube 2 Water + albumin solution + Biuret reagent
Test tube 3 Water + potato starch solution + Biuret reagent

Activity 3

Data Table 3

Test Tube ID	Observation
Water
Vegetable or olive oil
Vinegar
Salad dressing
Mustard or ketchup
Unknown A
Unknown B
Unknown C

 

2. In the quick test for fats (Activity 3), is there a relationship between the size of the spot and the fat content of the food? Explain your answer.

Activity 4

Data Table 4

Test Tube ID	Observation
Test tube 1a Albumin + pepsin + HCl + Biuret reagent before incubation
Test tube 2a Albumin + pepsin + water + Biuret reagent before incubation
Test tube 1b Albumin + pepsin + HCl + Biuret reagent after incubation
Test tube 2b Albumin + pepsin + water + Biuret reagent after incubation

 

3. In which test tube(s) did protein digestion occur (Activity 4)? How do you know?

Activity 5

Data Table 5

Cup ID	Observations
Cup 1 Cracker + distilled water + iodine-potassium iodide
Cup 2 Cracker + saliva + iodine-potassium iodide

 

4. What do you think would happen if the saliva treatment of the starch (cracker) was conducted under very cold conditions? Very hot conditions?

 Photos

Photo 1

Insert the photo from Activity 1, step 5. The following should be indicated in this photo:

• Test Tube 1 – water
• Test Tube 2 – starch
• Test Tube 3 – albumin

 Photo 2

Insert the photo from Activity 1, step 5 for your additional food samples. Indicate which food is in which test tube.

Photo 3

Insert the photo from Activity 1, step 5. The following should be indicated in this photo:

• Test Tube 1 – water
• Test Tube 2 – albumin
• Test Tube 3 – starch

 Photo 4

Insert the photo from Activity 2, step 5 for your additional food samples. Indicate which food is in which test tube.

 Photo 5

Insert the photo from Activity 3, step 8 of your group of fat test samples. Indicate which spot is for which food.

 Photo 6

Insert the photo from Activity 4, step 6, The following should be indicated in this photo:

• Test Tube 1a
• Test Tube 1b
• Test Tube 2a
• Test Tube 2b

 Photo 7

Insert the photo from Activity 4, step 11, The following should be indicated in this photo:

• Test Tube 1a
• Test Tube 1b
• Test Tube 2a
• Test Tube 2b

 Photos 8 & 9

Insert the photos from Activity 5, step 9. The following should be indicated in these photos:

• Cup 1
• Cup 2