DNA Replication, RNA Transcription, and Protein Synthesis Dry Lab

Activity 2

 Write down the mRNA sequence for:

 

  1. met-ser-leu-gly-trp-stop

 

met ser leu gly trp stop
           

 

  1. met-ser-pro-cys-his-stop

 

met ser pro cys his stop
           

 

  1. met-ile-asn-ala-tyr-stop

 

met ile asn ala tyr stop
           

 

  1. met-gln-thr-leu-arg-cys-pro-lys-stop

 

met gln thr leu arg cys pro lys stop
                 

 

  1. start-ile-glu-asp-ser-his-trp-stop

 

start ile glu asp ser his trp stop
               

 

  1. start-leu-pro-gln-tyr-ile-met-val-ala-stop

 

start leu pro gln tyr ile met val ala stop
                   

 

  1. start-ala-glu-trp-ser-phe-pro-ser-gly-thr-stop

 

start ala glu trp ser phe pro ser gly thr stop
                     

 

  1. start-val-ala-thr-thr-leu-tyr-cys-gly-arg-stop

 

start val ala thr thr leu tyr cys gly arg stop
                     

 

  1. start-lys-asn-gly-phe-his-thr-arg-pro-gln-stop

 

start lys asn gly phe his thr arg pro gln stop
                     

 

  1. start-met-thr-asn-lys-pro-gln-ser-leu-arg-stop

 

start met thr asn lys pro gln ser leu arg stop
                     

Activity 3

Step 1, insert picture and explain the results.

Step 2, insert picture and explain the results.

Step 3, insert picture and explain the results.

Step 4, insert picture and explain the results.

Step 5, insert picture and explain the results.

Step 6, insert picture and explain the results.

Step 7, insert picture and explain the results.

Step 8, insert picture and explain the results.

Step 9, insert picture and explain the results.

Step 10, insert picture and explain the results.

 

Activity 4

Simulate 3 different point mutations, 3 different insertion mutations, and 3 different deletion mutations starting from the original sequence of amino acids and explain why the protein product stays the same after some point mutations, but changes after others. Take pictures of the screen for all nine mutations and insert them below. Describe the mutation and explain the effect on the peptide.

  • Point mutation 1
  • Point mutation 2
  • Point mutation 3
  • Insertion mutation 1
  • Insertion mutation 2
  • Insertion mutation 3
  • Deletion mutation 1
  • Deletion mutation 2
  • Deletion mutation 3

 

Activity 5

Step 1: Design a protein product that consists of mainly hydrophilic amino acids. Take pictures of the screen and insert them on the lab manual template.

 

Step 2: In order for a protein to be exported out of the cell, the head needs to be hydrophobic. Design a protein with a hydrophobic head of at least four amino acids. Take pictures of the screen in the simulation of the protein you have designed and insert them on the lab manual template.

  • Why does the protein product stay the same after some point mutations, but change after others?