Perform a separation of plant pigments using thin-layer chromatography. Determine the relative polarity of plant pigments based on the Rf value of the TLC plate. Calculate Rf values of plant pigments. Explain how thin-layer chromatography works.

Thin Layer Chromatology Lab 6

Overview
In this investigation, students will extract pigments from spinach, then perform thin-layer chromatography to identify the pigments using two solvent solutions and the Rf value of the pigment.

Outcomes
• Perform a separation of plant pigments using thin-layer chromatography.
• Determine the relative polarity of plant pigments based on the Rf value of the TLC plate.
• Calculate Rf values of plant pigments.
• Explain how thin-layer chromatography works.