Effect of pH, temperature and lactose concentration on lactase activity

In this activity, you will study the effect of pH, temperature and lactose concentration on lactase activity. By the end of this activity, you should be able to:

  • Design experiments to study the effects of pH, temperature, and substrate concentration on enzyme activity.
  • Graph data related to enzyme activity.
  • Analyze data related to enzyme activity.
  • Complete a lab write-up summarizing and analyzing your results for the effect of temperature on lactase activity.
  • Identify the components of the scientific method.
  • Identify components of the substrate-enzyme-product system being studied.