Thin Layer Chromatology Lab 6

Overview
In this investigation, students will extract pigments from spinach, then perform thin-layer chromatography to identify the pigments using two solvent solutions and the Rf value of the pigment.

Outcomes
Perform a separation of plant pigments using thin-layer chromatography.
Determine the relative polarity of plant pigments based on the Rf value of the TLC plate.
Calculate Rf values of plant pigments.
Explain how thin-layer chromatography works.