CHAPTER 11
BIOTECHNOLOGY :PRINCIPLES AND PROCESSES
1.Observe the figure given below :
- (a) Identify the process
- (b) Write the steps of this process 1 , 2 and 3
- (c) What is a primer?
- (d) Name the enzyme involved in this process .
- (e) Give its use in r-DNA technology.
2.Given diagram shows a technique used in r-DNA technology.
- (a) Identify the technique
- (b)What is the purpose of this technique in r-DNA technology ?
- (c) Name the stain used in this technique for visualizing DNA under UV-light.
- (d) How can you visualize the separated DNA fragments in this technique ?
- (e) What is elution ?
3..Observe the picture given below:
- (a) Identify the instrument in the figure.
- (b) Write any one use of this instrument.
- (c) Label A,B.C and D.
4.Observe the following figure:
- (a) Identify the figure.
- (b) What does (i) rop (ii) Bam HI
- (c) Identify the selectable markers present in it.
- (d)Explain the significance of ori’
5.The given figure shows a sequence of DNA that can be cut down by restriction endonuclease.
- a) Name the sequence.
- b) Write down the name of restriction endonuclease, which recognises the above sequence.
- c) Give its peculiarity.
7.How can we make a cell competent to receive a foreign gene or DNA ?
8.Match the items of column A with B A B
- a) Cloning vector (i) Bioreactor
- b) Separation of DNA fragments (ii) Taq polymerase
- c) PCR (iii) Eectrophoresis
- d) Converts raw materials into specific products.
(iv) Hind II
(v) p BR 322
9.Recombinant DNA technology is a complex process which involves several steps. Write down the major steps in recombinant DNA technology?
10. The discovery of restriction endonuclease is considered as milestone in the history of genetic engineering.
- a) What are restriction enzymes ?
- b) Which is the first discovered restriction endonuclease ?
11.Autonomously replicating circular extra-chromosomal DNA is?
12.Differentiate endonuclease and exonuclease.
