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Laboratory Portfolio Questions for A11IND 2019-2020

These questions form the basis of your A11IND coursework, the laboratory portfolio. You will need to provide data from your recent practical classes as well as answer specific questions designed to assess your application of knowledge. Please provide answers to all questions in ONE word document for submission. Materials and Methods etc. are not needed.

It is expected that you use your own images taken from the practical sessions. Any information (including figures and diagrams) that you use in your answers, that are not from your own data, need to be appropriately referenced or acknowledged.  All coursework should also be submitted through Turnitin before the submission deadline as indicated on Moodle.  This coursework contributes 40% of the final module mark.

 

Microscopy:

1. Describe the morphological features of bacteria stained by Gram staining technique with respect to their shapes and colours.  Explain the principles underlying this method when used in bacteria identification.  (4 marks)

 

 

1. Some bacteria cannot be stained or will not show adequate staining intensity using the Gram stain method.  Give two examples and for each example, explain the reason(s) why Gram stain fails to give good results (6 marks)

 

 

3. A sputum sample from a patient with tuberculosis has been sent to the pathology laboratory for testing. Describe the procedure to be used for visualising the pathogen responsible for tuberculosis in this sample (4 marks)

 

 

Pathogen identification:

1. Case scenario

A 32 year old male who has recently been to an Asian country develops projectile vomiting and profuse diarrhoea.  A stool sample has been received by the pathology department and the initial investigation on the sample has produced the following results:

 

 

Sample ID: S3

 

Culture:

Small colourless (transparent) colonies on McConkey agar

 

Heavy growth of yellow colonies on TCBS (Thioglycolate Citrate Bile Salt) agar

 

Gram stain results

S3 Yellow colony:  Gram negative curved rods

Gram positive control: Gram positive cocci present

Gram negative control: Gram negative bacillus present

 

Special staining for flagella:

S3 Yellow colony: Flagella present

Positive control: Flagella present

Negative control: Flagella not found

 

Motility test:

S3 yellow colonies: darting motility observed

Positive control: motile

Negative control: non-motile

 

Oxidase test:

S3 Yellow colony: Oxidase positive

Oxidase positive control: Oxidase positive

Oxidase negative control: Oxidase negative

 

Glucose test:

S3 Yellow colony: Glucose positive

Glucose positive control: Glucose positive

Glucose negative control: Glucose negative

 

 

1. Based on the information provided from the initial laboratory investigation, demonstrate the elucidation of the possible identity of the yellow colonies on the TCBS agar with stepwise explanation and list the resources used in the process of identification (5 marks)

 

 

 

2. Describe the difficulties in identifying the pathogen based on the initial laboratory findings and how to improve the identification process (4 marks)

 

 

3. Explain how the experimental processes were validated during the initial laboratory investigation of S3 (6 marks)

 

 

4. Explain how bacteria like S3 exerts its pathological effects on human gastrointestinal tract. A diagram may be used to support your answer (5 marks)

 

Latex agglutination:

 

1. What would be the benefit of using IgM for agglutination reactions performed in vitro? (3 marks)  When considering the serological status of a patient, if you detect the presence of IgM antibodies to an antigen, what might that indicate? (1 mark)

 

 

2. Explain the steps involved in the genetic testing of a micro-organism that would be performed to confirm its identity (4 marks)

 

Antimicrobial action:

1. Use the class data set provided on Moodle and indicate whether the organisms tested are considered susceptible or resistant to the antimicrobials which they had been tested against. Refer to sensitivity testing tables 1 & 2 when you perform this task. (6 marks)

 

 

S1
Antibiotic tested	Inhibition zone diameter class median (mm)   And     Acceptable range (mm)	Result interpretation S = sensitive R = Resistant	Comments
Ampicillin
Chloramphenicol
Tetracycline
Sulphafurazole	NA	NA	NA
Streptomycin	NA	NA	NA
Penicillin
Cefoxitin
Erythromycin

 

 

 

S2
Antibiotic tested	Inhibition zone diameter class median (mm)   And     Acceptable range (mm)	Result interpretation S = sensitive R = Resistant	Comments
Ampicillin
Chloramphenicol
Tetracycline	NA	NA	NA
Sulphafurazole	NA	NA	NA
Streptomycin
Penicillin	NA	NA	NA
Cefoxitin
Erythromycin	NA	NA	NA

 

 

2. Describe Penicillin’s mode of action with the aid of a diagram with emphasis on how it interferes with bacterial cell wall integrity (4 marks).

 

 

 

3. Explain why some organisms such as E coli are generally resistant to penicillin (2 marks).

 

 

4. When a bacterial strain such as S1 is resistant to amoxicillin, what is the possible mode of action of the gain in resistance by the bacteria (3 marks)? Are there options which can be considered to replace amoxicillin for an effective treatment to cure the infection (3 marks)?

 

 

Total marks = 60

The Brief for Assignment

This Assignment 2 is an individual work. It is a continuation of Assignment 1 and based on the same property located at 60-70 Elizabeth Street, Sydney. The heritage building was completed in 1929/30, with major refurbishment over 1985 – 1988 and a substantial foyer upgrade in 2004. Whilst Assignment 1 is related to property market analysis and strategic asset management plan, this Assignment extends your knowledge and skills to sustainable property management in the office sector.

As the property management consultant, you are required to submit a business case report to introduce a sustainable property management strategy for improvement of the operating and asset performance of this existing property to the property owner. Along with all other relevant matters, this ‘sustainable property management report’ is to include the following:

1. Discuss how the sustainability concept is being implemented within the office property sector globally and in Australia during the last decade, highlighting the trends, achievements and current stage in the implementation of sustainability to existing buildings.
2. Evaluate the property owner’s and the tenant’s views towards sustainable property.
3. Recommend what environmental improvement and property management measures should be adopted for this property to achieve such benefits. Make all necessary assumptions in relation to the building design, construction and existing sustainable performance.
4. Examine how the possible barriers of introducing such measures to this property can be overcome, addressing the concerns of the property owner and tenants.
5. Salient facts of sustainable property management practice in existing buildings and conclusions on the proposed green measures for this property.

Length of the final report: 10 pages (2000 words); you may extend this limit to 12 pages.

NB:

1. • All arguments should be properly cited in the reports and a list of all references that you have used should also be given.
   • Assignment length limits are defined in terms of pages. This limit includes illustrations and references. The page size must be A4 and the font size for text should be 12-point Times Roman or equivalent. Use a wide margin and one and half spacing.
   • Use the attached Cover Sheet when the report is submitted.

Needs to be set out with exec summary with the opinion on the research. Coverage in the report should be purely legal points.

Areas to address are:
– development of ADR in family cases and the rationale for placing a greater emphasis on the process
– Options available for clients pursuing ADR in UK and how this compares to other jurisdictions (such as USA and Poland, or another European country)
– Review of literature to assess pros and cons for clients of ADR in family cases
– impact of the emphasis on ADR on the work of family legal practices and approach to the court.

Needs to be OSCOLA referencing

Essay – “How Your Element Related to Energy”

In celebration of the International Year of the Periodic Table, the theme of “How Your Element Related to Energy” is to ask all students to produce a short article (within two A4 page and flexible format) on one element of the periodic table. The benefits to our students include:

• Based on the teamwork presentation (self-assessment but not counting to the final marks), the basic knowledge on one element group of Periodic Table have been explored. More deep insights on the theme area can be expected.
• Working on employability skills, such as data analysis, teamwork and communication.
• Work on communication and report-making skills.
• The chance to have their work contribute to the sociality and related industries.
• You have broad options for the contents, such as manufacturing, use and disposal of a certain element (or related chemicals).

Assessment Criteria 

• Covers the most important aspects of the subject thoroughly
• Shows the understanding of fundamental concepts with evidence of critical analysis
• Well-presented using appropriate academic conventions
• Uses and collates information/data from relevant and appropriate sources
• Written clearly and concisely; showing originality and well-presented reasons for your choice of element

 

Here is the assignment brief.

Module title	Political Journalism
Assessment title	Essay
Assessment task details and instructions   The essay should address one of the following topics :   ·         Assess the relevance of political journalism in public service media provision   ·         Critically evaluate the significance of media convergence for approaches to, and expectations of, political journalism   ·         To what extent and how has UK political journalism assisted the public debate on EU withdrawal?   Assess the impact of the lobby system in political journalism   Assess the impact of social media in election campaigns   ·         Analyse the role of the spin doctor in political journalism   These are mainly very broad topics so you will need to apply the general principles to a limited number of specific case studies
Assessed intended learning outcomes   On successful completion of this assessment, you will be able to: demonstrate an awareness of the UK lobby system and its relation to political reporting critically analyse the use of social media in political reporting demonstrate an understanding of election reporting in both the UK and US and/or show a critical awareness of the role and relationships of the reporter, politicians and press officers
Module Aims •          Develop a critical understanding of the challenges, constraints and influences facing journalists engaged in political reporting; •          Evaluate the ethical dilemmas involved in political reporting and in particular the pressures around editorial control, the lobby system and spin; •          Deploy professional skills including original research and interviewing to produce an original piece of journalism including political interviews; •          Demonstrate an informed ability to critically evaluate your own work; •          Demonstrate an analysis of political campaigns and the contrasting system in the US.
Word count/ duration 2,200 words The word count includes all citations but not the Bibliography.
Assessment Criteria Marks for your assessment will be allocated based on ·         Understanding of political journalism in regional, national and international contexts ·         Critical analysis of political processes ·         Ability to engage the reader and present an argument ·         Evidence of engagement with wider reading You should consult Blackboard for detailed grade or mark descriptors.