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Why does glucose uptake by a skeletal muscle cell require a transporter such as GLUT4? In other words, why isn’t simple diffusion possible? What stimulates the insertion of GLUT4s into the sarcolemma?

NATIONAL CENTER FOR CASE STUDY TEACHING IN SCIENCE “I Hate Running!” by Rhodes, Rozell, & Wilson

Part I — Blood, Skeletal Muscle Cells, and Glucose Uptake

To untangle the misconceptions in the conversation between Shelby and Patrick, let’s begin by looking at the structures
within skeletal muscle cells that are involved in energy production at a submaximal level of exercise in an untrained

person. This would be the case for Shelby, who is just beginning an aerobic exercise program that includes mostly

endurance training occurring in 20–30 minute bouts. In addition, let’s examine some common metrics such as lactate

threshold and blood pH to assess what is happening in an untrained person during exercise.

During the first 30 minutes of Shelby and Patrick’s run at a submaximal level (40–70% VO
2 max), blood glucose levels
increase due to glucose release from the liver (Zinker
et al., 1990), providing skeletal muscle cells with a concentration
gradient of glucose for uptake and subsequent ATP production. However, glucose is a large polar molecule and cannot

simply diffuse unaided into the sarcoplasm through the phospholipid bilayer comprising the sarcolemma of skeletal

muscle cells. Instead, to take up blood glucose, skeletal muscle cells must produce and insert glucose transporters,

commonly referred to as GLUTs, into their sarcolemma. These GLUTs are a diverse family of large, integral membrane

proteins that permit facilitated diffusion of glucose down its concentration gradient. Skeletal muscle cells can express

several different forms of GLUTs, but the most common form is GLUT4 (Gaster
et al., 2000; Goodwin, 2010;
Richter & Hargreaves, 2013). Interestingly, the insertion of GLUT4 into the sarcolemma can either be stimulated by

insulin binding to its receptor on skeletal muscle cells that are resting, or by an insulin-independent mechanism during

contraction of the skeletal muscle cells (Richter & Hargreaves, 2013).

Activity 2
Using the information above, complete the flow chart (Figure 1) on the following page to get a better understanding

of the sequence of events that occurs during exercise. Then label all structures shown in the diagram (Figure 2), which

represents a portion of a skeletal muscle cell’s sarcolemma during exercise. Also label the extracellular fluid compart

ment, blood, and sarcoplasm. Indicate where in the body glucose and insulin would have come from and how they are

transported to the skeletal muscle cell.

Questions
1. Why does glucose uptake by a skeletal muscle cell require a transporter such as GLUT4? In other words, why isn’t

simple diffusion possible?

2. What stimulates the insertion of GLUT4s into the sarcolemma?

3. The existence of GLUT4s in the sarcolemma does not guarantee glucose uptake into the cell. Why? What else is
required?

4. Exercise helps reduce blood glucose levels in people, even if they are insulin resistant and thus their cells can no
longer respond to insulin efficiently. How is this possible?

Explain the mechanism of NAFLD and NASH (mention second hit theory/oxygen species/inflammatory cytokines). Is NASH more life threatening than NAFLD based on its association with Cirrhosis? 

Learning Topic 1: Inherited  Metabolic Disorder

 

 Question: Is tetrahydrobiopterin (BH4) useful in managing patient with Phenylketonuria?/ PKU?

 

(Use references in havard style and own words, explain what PKU condition is, talk about phenylalanine hydroxylase deficiency and BH4 in PKU. Remember not all patients are responsive to BH4 treatment.)

 

Summarise the paper below and explain why you chose the article/paper and also whether you consider the data in those paper represents a small or a significant advance in your opinion. Look at the example document provided.

 

References :

 

  1. https://academic.oup.com/jcem/article/81/2/470/2649344

 

 

  1. https://www.sciencedirect.com/science/article/pii/S1096719219303634?via%3Dihub
  2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911353/

 

 

 Learning Topic 2 : Liver in Health and Disease

 

 

 

Key Question: Explain the mechanism of NAFLD and NASH (mention second hit theory/oxygen species/inflammatory cytokines). Is NASH more life threatening than NAFLD based on its association with Cirrhosis?  Use references and use your own words. Do not copy references or words from the example.

 

Paper References

 

 

  1. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5339475/
  2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4943559/
  3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7098369/

 

 

Learning topic 3: Lipids and Lipoprotein Metabolism

 

 

Question:  Is omega-3 fatty acids (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) or statins an effective treatment in reducing cholesterol level and preventing cardiovascular disease? Give your reasons with references. Please use references and write in your own words.

 

 References:

 

  1. https://onlinelibrary.wiley.com/doi/10.1111/joim.13016
  2. https://onlinelibrary.wiley.com/doi/full/10.1111/j.0954-6820.1985.tb08887.x
  3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389556/

 

How many individuals are included in the data set? Is the left-right ideology similar on average for those who did and not answer the item NatFrEst? Calculate the median of the variable NatFrEst. What does this tell us about the distribution of perceptions about fraudulent benefits claims?

Final Coursework

Quantitative Data Analysis (POLS0083)

Instructions

The final assessment will be posted on Moodle on 17th December 2021 at 6pm, and is due on 10th January 2022 at 2pm. Follow all designated SPP submission guidelines for online submission as detailed on the POLS0083 Moodle page. Standard late submission penalties apply.

This is an assessed piece of coursework (worth 70% of your final module mark) for the POLS0083 module; collaboration and/or discussion of the coursework with anyone is strictly prohibited. The rules for plagiarism apply and any cases of suspected plagiarism of published work or the work of classmates will be taken seriously.

As this is an assessed piece of work, you may not email/ask the course teaching team questions about the coursework.

Along with the coursework questions, the necessary data sets for the coursework can be found on the POLS0083 page on Moodle.

The coursework should be submitted via the ‘POLS0083 – 2,000 word Final (70%)’ link on the course Moodle page. You will need to click the ‘Submit Paper’ link at the bottom of the page. When presented with the ‘Submit Paper’ box, the ‘Submission Title’ should be your candidate number, and you should upload your document into the box provided. Remember to state ONLY your candidate number on your coursework (your candidate number is made up of four letters and one number e.g. ABCD5). Your name and/or student number MUST NOT appear on your submission.

The coursework consists of three separate sections, each with several questions. The marks allocated for each section are indicated in the text. You must complete each question to achieve full marks.

Unless otherwise stated, answers should be written in complete sentences. Be sure to answer all parts of the questions posed and provide a substantive interpretation of the results.

The word count for this assessment is 2,000 words. This does not include the code, your output, or any words (or numbers) contained within tables or figures.

Submit your type-written (numbered) answers in a single document (a word file or a pdf file). You can, but do not have to, use RMarkdown to produce such a file. If you use RMarkdown, you can integrate the code with the answers (make sure that it is completely visible). If you do not use RMarkdown, you can create an appendix section at the end which contains all the R code needed to reproduce your results. Your code has to work when we run it. You do not need to include the code that failed to run, but just the well-annotated, cleaned-up version. If you do not provide the code to a
question, any written answer to that question will be disregarded.

You may assume the methods you have used (e.g. difference in means, linear regression, etc) are understood by the reader and do not need definitions, but you do need to be able to explain what they do and how they apply to answering the question.

Round all numbers to two digits after the decimal point.

Do not screenshot or copy and paste any brute R output (e.g. lm(y x)) into your answers. Create a formatted table that is easy to read.

Assign every table and figure a title and a number and refer to the number in the text when discussing a specific figure or table

Section 1
Public perceptions of benefit fraud
Trust in social welfare institutions relies on how the public perceives of the deservingness of recipients of such benefits. In particular, notions of so-called ‘benefit cheats’ erodes public confidence in social welfare. Past surveys have shown that the public tend to greatly over-estimate the financial scale of benefit fraud. For example, a 2013 Ipsos MORI study found that on average, respondents guessed that £24 out of every £100 in benefit claims is done fraudulently, whereas the official estimate is around £0.70 of every £100.

In this section, we explore public perceptions about fraudulently claiming benefits, and in particular, about how widespread false benefits claims are. More specifically, we will examine whether certain socio-demographic characteristics are associated with public perceptions about how widely false benefit claims are made in the UK.
We will use part of the British Social Attitudes (BSA) data set on poverty and wealth, which you can download as bsa-poverty.csv from the POLS0083 Moodle page. The data set contains the following variables:

Variable name Description

NatFrEst Answer to the question “Out of every 100 people receiving benefits in Britain, how many have broken the law by giving false information to support their claim?”

leftrigh Five-point left-right ideological scale, with 0 to the left and 4 to the right RSex Sex of respondent, 1 if male or 0 if female HEdQual3 Completed university degree, 1 if the respondent completed degree or 0 if not

You can load the data set by using the following command: bsa <- read.csv(“data/bsa-poverty.csv”)

Questions (42 Marks)
1. How many individuals are included in the data set?

2. Is the left-right ideology similar on average for those who did and not answer the item NatFrEst?

3. Calculate the median of the variable NatFrEst. What does this tell us about the distribution of perceptions about fraudulent benefits claims?

4. Create a histogram for NatFrEst and interpret it. What does this tell us about public perceptions about fraudulent benefits claims?

5. We are interested in seeing whether there is a relationship between a person’s left-right orientation and how widespread they think fraudulent benefit claims are. Fit the relevant simple linear regression model and interpret the substantive significance of the estimated slope coefficient. You do not need to discuss statistical significance.

6. State a null and an alternative hypothesis for the estimated slope coefficient, decide whether to reject the null hypothesis, and provide a conclusion.

7. How is your conclusion in Question 6 related to Type I and Type II error?

8. We now add whether the respondent completed a university degree and respondent sex to our analysis. Interpret the estimated coefficient for the left-right orientation. Does your answer to the question about the relationship between a person’s left-right orientation and NatFrEst change? If so, how?

9. Calculate and interpret the 99% confidence interval for the estimated coefficient for completing a university degree from the model you fitted in Question 8. What does the estimated standard error tell us?

10. Finally, we are interested in whether the relationship between left-right orientation and perceptions of how widespread benefit fraud is also depends on an individual’s education level.
a. Add the relevant interaction term to your regression model and display your results.
b. Assess the model fit for this regression model.
c. Interpret the estimated interaction term and discuss its substantive and statistical significance.
d. Interpret the intercept and its statistical significance. Is the intercept meaningful in this regression model?
e. Visually represent the results from the regression model using four lines (one each for male respondents with a university degree, female respondents with a university degree, male respondents without a university degree and female respondents without a university degree) and describe what your graph shows.

Section 2

Dystopian fiction and willingness to justify radical political action

We now look at part of the recent study by Jones and Paris (2018), who conducted a series of survey experiments to examine whether exposure to dystopian fiction affects an individual’s political beliefs. They define dystopian fiction as “portray[ing] a dark and disturbing world dominated by an overwhelmingly powerful. . . controlling entity that acts to undermine core values”. In particular, the authors hypothesised that exposure to dystopian fiction would lead to higher support for more radical political action, especially for more violent action.
In the first study, the authors randomly assigned the US-based respondents into two groups. Respondents assigned to the treatment group first read an excerpt from the first book in Hunger Games, and then watched a 17-minute video with various violent scenes from the Hunger Games films. Respondents assigned to the control group were not exposed to any media.
The premise of Hunger Games is that an overwhelmingly powerful government forces individuals to take part in a contest where the participants are forced to kill each other until only one survivor remains.
The outcome variables are a series of attitudinal items about willingness to justify different disruptive activities: civil disobedience, damaging government property, cyber attacks on government websites, armed rebellion, and violent protest.

The data file you will use, which can be downloaded on the POLS0083 Moodle page, is titled dystopia.csv.
The data includes the following variables:
Variable name Description hgindic Treatment group, with 1 for those exposed to Hunger Games and 0 for the
control group
j_disobed How much civil disobedience can be justified, on a 0-1 scale
j_damage How much damaging government property can be justified, on a 0-1 scale
j_cyber How much cyberattacks on government websites can be justified, on a 0-1 scale
j_rebel How much armed rebellion can be justified, on a 0-1 scale
j_violent How much violent protest can be justified, on a 0-1 scale
female Respondent sex, with 1 as female and 0 as male ideo Respondent left-right ideological orientation, with higher scores indicating the right, on a 1-6 scale
You can load the data set by using the following command: dystopia <- read.csv(“data/dystopia.csv”)

Questions (33 Marks)
1. What are the proportions of female respondents within the treatment and control groups?
2. We are interested in the outcome variables for civil disobedience and armed rebellion. For each of these outcome variables:
a. Create a boxplot for each treatment group and interpret the results.
b. Calculate difference in means by treatment group and interpret the results. You do not need to discuss statistical significance.
c. Conduct the appropriate hypothesis test and interpret the results for the difference in means.

3. In the current study, in which two ways can we understand the population from which the sample is drawn?

4. With what assumptions can we interpret the results from Question 2 as causal?
5. Fit a regression model for each outcome variable with the treatment group, sex, and ideology. Does the estimated difference in means change? What does this tell us about the randomisation of treatment in this study?
6. Do your results support the authors’ hypothesis about the link between dystopian fiction and willingness to support radical political action? State your conclusions in terms of substantial and statistical significance.

Section 3

Direct democracy and citizens’ local support
Does direct democracy (such as local referendums) increase satisfaction with local policies and governmental institutions? Marien and Kern (2018) explore this question in their article “The Winner Takes It All:
Revisiting the Effect of Direct Democracy on Citizens’ Political Support”.
Drawing on the existing literature, the authors argue that direct democratic instruments have short-term
positive effects on satisfaction with local policies and institutions, since they increase the perceived fairness of
decision-making and ability to influence political outcomes among the electorate.
To test their hypothesis, the authors conducted multiple surveys during the spring of 2015, when the
Belgian city Mechelen conducted a referendum on traffic circulation, after citizens had objected to the local
government’s initial plans. The researchers collected survey data before and after the referendum took place
both from a sample of local residents in Mechelen who lived in neighbourhoods affected by the traffic diversion
plans (treatment group) and from a sample of residents of a similar neighbourhood in Mechelen not affected
by the traffic circulation plans (control group).

The data file you will use, which can be downloaded on the POLS0083 Moodle page, is titled referendum.csv.

The data includes the following variables:
Variable name Description
idresp Respondent ID
neighb Neighbourhood, 1 as the treatment group and 0 as the control
w1_byear Respondent birth year
primary Highest level of education completed – primary – with 1 Yes and 0 No
secondary Highest level of education completed – secondary – with 1 Yes and 0 No
tertiary Highest level of education completed – tertiary – with 1 Yes and 0 No
w1_trust_general General level of trust on a 0-10 scale, with higher scores meaning more trust, before the referendum
w1_pol_interest Political interest on a 0-10 scale, with higher scores meaning more interest, before the referendum
w1_democracy_satis Democratic satisfaction on a 0-10 scale, before the referendum, with higher scores meaning higher satisfaction
w2_democracy_satis Democratic satisfaction on a 0-10 scale, after the referendum, with higher scores meaning higher satisfaction
You can load the data set by using the following command: referendum <- read.csv(“data/referendum.csv”)

Questions (25 Marks)
1. Is the average level of democratic satisfaction before the referendum significantly different (statistically and substantively) between respondents in the treatment and control groups?

2. Provide some evidence that the treatment and control neighborhoods are similar in terms of respondent characteristics before the referendum took place.

3. Focusing on the respondents living in a neighborhood affected by the government’s plan on traffic circulation, use a before-after design to estimate the average treatment effect. Is this difference significant both statistically and substatively?
4. With what assumptions can we consider the results of Question 3 as causal?

5. Calculate and interpret the difference-in-differences for democratic satisfaction for the two neighborhoods and interpret your results.

6. Do the results in Question 5 support the authors’ hypothesis about the link between direct democracy and citizens’ perceptions about democracy? Why or why not?

7. With what assumptions can we consider the results of Question 6 as causal?

Why is it so important for a company to delineate and appraise its strategy?

How to Evaluate Corporate Strategy

Read the article on How to Evaluate Corporate Strategy and then write a one to three sentence summary of each of the sections below and what they mean to creating one’s corporate strategy (1-6 below). Then, answer the question at the bottom of this assignment (Q below).

1. Internal consistency.

2. Consistency with the environment.

3. Appropriateness in the light of available resources.

4. Satisfactory degree of risk.

5. Appropriate time horizon.

6. Workability.

Q: Why is it so important for a company to delineate and appraise its strategy?

Pick two non-psychodynamic approaches; describe these showing how they can supplement a psychodynamic approach in work with children.

Psychodynamic and Non-psychodynamic

Pick two non-psychodynamic approaches; describe these showing how they can supplement a psychodynamic approach in work with children.

1. Therapeutic Children’s Homes :

Raising the bar? Evaluation of Social Pedagogy pilot programme in Residential Children’s Homes : Department of Education (Research Report DFE-RR14)

A coat of many colors” in The Arts in Psychotherapy

Article by Ephrat Huss Vol 33, Issue 3, July2009-7( P154-160)

Care and Youth Review Services: Vol 34, Issue 2, Pages 437-450

In, out and after care: Young adults’ views on their lives, as children, in a therapeutic residential establishment by Bernard Gallagher; Adam Green 02/2012

2. Disable Children’s rights, Shift from deficit approach, Critical Disability studies

Draw on Children’s rights and Critical Disabilities studies, prioritising both disabled children’s voices and an approach which is grounded in respect, fun and the UNCR support on the right to play and leisure.

Disability & Society: Disabled children’s childhood studies: a distinct approach? Article by Tillie Curran & Katherine Runswick-Cole

– Include all article provided in the essay if you can when referencing.

Define and discuss what collective bargaining is. Research, outline, and discuss all of the steps of the collective bargaining process. Research, outline, discuss, and evaluate the best practices for collective bargaining strategies.

WEEK 4 348 ASSGIMENT

You are the president of the Local Union 312 Chapter. As the president of the union, you are the chief spokesperson and representative of the Local Union 312 to management. Next month, you will start the collective bargaining process with management to get your union members greater wages and benefits and better and safer working conditions. Write a 3–5-page research paper using APA style outlining the collective bargaining process, including the following:

Define and discuss what collective bargaining is.
Research, outline, and discuss all of the steps of the collective bargaining process.
Research, outline, discuss, and evaluate the best practices for collective bargaining strategies.
Research, outline, discuss, and evaluate the arguments against and for collective bargaining.

Use correct APA style, grammar, sentences, and punctuation.
Support your research paper with at least 4 different scholarly sources, such as research journals, research studies, and government or accredited educational institutions’ Web sites.

Why is the ACLU so concerned about this issue? Summarize two current issues or press releases. What is your reaction? What are the most important things that citizens should know regarding that right? Did anything you learned about this topic surprise you?

Internet Activity 2

In this assignment, you will investigate information concerning civil liberties and rights by looking at current issues involving the rights of individuals as presented by the American Civil Liberties Union and the Institute for Justice. Do NOT cut and paste your answers from these websites. Summarize and respond in your own words.

Directions
Begin by going to the ACLU website.

What is the mission of this organization?

Click on ‘Issues’. You will see several campaigns listed. Choose one and read the background material, including some of the additional resources (Current Issues and The Latest news/press releases.) Why is the ACLU so concerned about this issue? Summarize two current issues or press releases. What is your reaction?

Click on ‘Know Your Rights’ and choose an area that interests you. What are the most important things that citizens should know regarding that right? Did anything you learned about this topic surprise you?

Click on ‘Defending Our Rights’. Read and summarize 2 cases that the ACLU is involved. Do you agree with the ACLU’s position on those cases? Why or why not?
Next, go to the Institute for Justice.

What is the mission of this organization? What kinds of issues are they most concerned about?
Under ‘Issues’ in the menu bar, choose ‘First Amendment’ and one other category. Summarize and react to at least one case from each category.

After reviewing this material, how would you assess the efforts of these two organizations?

Do you feel that the Bill of Rights has been violated or upheld? Explain your reasoning in some detail. Make sure to provide proper source information for the stories you decide to discuss!

Internet Activity 1

Navigate to Current Events on the Bill of Rights Institute website.

Summarize a minimum of three articles. Explicitly state the way(s) in which they relate to the Bill of Rights. Do NOT cut and paste from articles. Summarize in your own words.
In each of these stories, do you feel that the Bill of Rights has been violated or upheld? Explain your reasoning in some detail. Make sure to provide proper source information for the stories you decide to discuss!

Answer the questions in Microsoft Word in a paper of about 2 pages. Provide a citation for the articles you read in APA format.

Define the four basic principles of ethical research: autonomy, beneficence, nonmaleficence, and justice. Then explain how these four principles interrelate in the conduct of ethical research.

Ethical research

1. Define the four basic principles of ethical research: autonomy, beneficence, nonmaleficence, and justice. Then explain how these four principles interrelate in the conduct of ethical research.

2. Explain the importance of conducting ethical research.

3. In addition to the most current APA Ethical Principles of Psychologists and Code of Conduct, select one other ethics code from a professional social science association (preferably a different one than used in Written Assignment 3, I used Evaluation of APA Ethical Principles of Psychologist and Code of Conduct and National Association of Social Workers in Assignment 3 so don’t use those) Examine how both ethical codes interweave the four basic principles of ethics into their model for the appropriate conduct of research.

4. Select a research study that is different from the one you selected in Discussion Forum 4( The case is “Is Death a Dying Business?”. Sype (2018) in Discussion Forum 4 so DO NOT USE THAT ONE.) Then conduct an ethical analysis of the research study. Provide all pertinent identifying information along with the steps you made in conducting your analysis.

https://www.apa.org/ethics/code/

Discuss a labor law from your state. If you were to accept a position in another state, why do you believe it would be important to understand the state labor laws prior to arriving at your new position?

Week 4 348

Discuss the following in your main post:

The industrial relations principles are not designed to apply favorably to only one segment. The company, union, and employee perspective must be taken into consideration in the grievance process. Discuss each of these perspectives.
Whether the differences are relatively minor or vastly different, labor laws can vary based on the specific state law.

Discuss a labor law from your state. You can use this site to assist you in your research.
If you were to accept a position in another state, why do you believe it would be important to understand the state labor laws prior to arriving at your new position?